PGK - NEO cassette
نویسندگان
چکیده
1. Abstract 2. Introduction 3. Methods and Materials 3.1. Generation of the targeting vector 3.2. Electroporation, selection and screening of embryonic stem (ES) cells 3.3. Blastocyst injection and animal breeding 3.4. Isolation of RNA, Quantitative PCR and whole-mount in situ hybridization 3.5. Skeletal X-rays and staining 4. Results 4.1. Generation of Zic3 4.2. Upregulation of Zic3 expression in the Zic3 allele 4.3. No embryonic lethality observed with Zic3 4.4. Left-right axis abnormalities and axial skeletal defects 4.5. Craniofacial anomalies, hyoid bone and cricoid cartilage defects 4.6. Ectopia Cordis 5. Discussion 6. Acknowledgement 7. References
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